منابع مشابه
Positive and negative regulation of insulin gene transcription.
Introduction The protein hormone insulin plays a decisive role in the control of mammalian glucose homeostasis. In adults, production of insulin is stringently restricted to the B cells of the endocrine pancreas. The major mechanism directing this specificity operates at the transcriptional level; as a result, insulin mRNA levels are extremely low in n0n-B cells, of the order of 10' lower than ...
متن کاملShort-term regulation of insulin gene transcription by glucose.
Whereas short-term regulation of insulin biosynthesis at the level of translation is well accepted, glucose-dependent transcriptional control is still believed to be a long-term effect occurring after more than 2 hr of glucose stimulation. Because pancreatic beta cells are exposed to elevated glucose levels for minutes rather than hours after food uptake, we hypothesized the existence of a shor...
متن کاملInsulin regulation of rat growth hormone gene transcription.
We have previously shown that insulin suppresses growth hormone (GH) messenger (m) RNA levels in rat pituitary cells. To further delineate the molecular mechanism of insulin action, the effect of insulin treatment on GH gene transcription rates was examined in GH3 pituitary cells grown in serum-free defined medium. A transcriptional run-off assay was performed when intact isolated nuclei were a...
متن کاملRegulation of ribosomal DNA transcription by insulin.
The experiments reported here used 3T6-Swiss albino mouse fibroblasts and H4-II-E-C3 rat hepatoma cells as model systems to examine the mechanism(s) through which insulin regulates rDNA transcription. Serum starvation of 3T6 cells for 72 h resulted in a marked reduction in rDNA transcription. Treatment of serum-deprived cells with insulin was sufficient to restore rDNA transcription to control ...
متن کاملRegulation of insulin gene transcription by the immediate-early growth response gene Egr-1.
Changes in extracellular glucose levels regulate the expression of the immediate-early response gene and zinc finger transcription factor early growth response-1 (Egr-1) in insulin-producing pancreatic beta-cells, but key target genes of Egr-1 in the endocrine pancreas have not been identified. We found that overexpression of Egr-1 in clonal (INS-1) beta-cells increased transcriptional activati...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 2002
ISSN: 0012-186X,1432-0428
DOI: 10.1007/s00125-001-0728-y